Fluorescence camera HiCAM Fluo
HiCAM Fluo from Lambert Instruments is a particularly powerful yet compact fluorescence camera that is connected to fluorescence microscopes.
Its exposure time can be adjusted down to the nanosecond range to image even fastest phenomena. By using high-quality Gen III amplifiers (18 or 25 mm), imaging down to the detection of single photons is possible. The amplifiers can be adapted to the respective application (UV to NIR) by a number of possible photocathodes.
HiCAM Fluo can also be used as a high-speed shutter by gating. This reduces the effective exposure time and allows motion blur to be reduced. The fanless cooling system, with its virtually non-existent vibrations, also ensures an improved signal-to-noise ratio and thus optimum image quality.
Thanks to its compact aluminum housing, the HiCAM Fluo is very handy and, with the help of its universal connections, it can be used with basically any fluorescence microscope. The images are streamed directly via a CXP (CoaXPress) interface with four CXS connections (6.25 Gbit/s each). The associated software not only controls the camera and measurement parameters, but also data processing, amplifier power and gating.
Applications
- Combustion analysis
- Cell medicine
- Microbiology
- Chip manufacturing
- Microfluidics
- LIF (laser-induced Fluorescence)
- MTV (molecular tagging velocimetry)
Specifications
| Camera specifications | ||
|---|---|---|
| HiCAM Fluo 1000 | HiCAM Fluo 2000 | |
| Max. resolution | 1280 × 1024 pixels | 1920 × 1080 pixels |
| Frame rate | 1000 fps at max. resolution 1500 fps at 1200 × 720 pixels 4000 fps at 640 × 480 pixels 7500 fps at 256 × 256 pixels | 2000 fps at max. resolution 2500 fps at 1280 × 960 pixels 5100 fps at 640 × 480 pixels 6400 fps at 512 × 384 pixels |
| Pixel size | 6.6 µm | 10 µm |
| Pixel depth | 10 or 12 bit | 8 bit |
| Computer interface | Streaming CoaXPress | Streaming CoaXPress 2.0 |
| Integrated features | Gain and gain control Anode current limiter Internal trigger generator Shutter control |
|
| Gating properties | |||
|---|---|---|---|
| standard | optional | precision gating | |
| Gating pulse width range | 40 - 10 ns | <3 - 10 ns | 3 ns - 1 µs |
| Min. pulse width (jitter) | 40 ns (<250 ps RMS) | <3 ns (<80 ps RMS) | 3 ns (<250 ps RMS) |
| Pulse width increments | 10 ns | 10 ps | 250 ps |
| pulse repetition rate | 100 kHz | 300 kHz, 2.5 MHz in burst mode | |
| Intensifier properties | ||
|---|---|---|
| Image intensifier | Proximity-focused Gen III GaAsP (filmless) | |
| Photon gain (max.) | 36,000 lm/m2/lx | |
| Phosphor | P46 (P20, P43 or FS on request, see also their own specifications table) |
|
| Input Window | Borosilicate glass | |
| Spatial resolution (bare intensifier) | Up to 62.5 lp/mm | |
| Spectral responses of phosphors |  |
|
| 18 mm Gen III intensifier | 25 mm Gen III intensifier | |
| Effective area on input | 12.78 × 12.68 mm | 17.75 × 17.61 mm |
| Input window thickness | 5.5 mm | 6.0 mm |
| Phosphor characteristics | ||||
|---|---|---|---|---|
| Phosphor | Peak wavelength | Photons/electrons @5 kV | Decay time to 10 % | Screen efficiency (Optical/Electrical Watt) |
| P46 | 548 nm | 240 | 1.3 ms | 8.7 % |
| P43 | 530 nm | 55 | 300 ns | 1.8 % |
| FS | 513 nm 668 nm 768 nm | 96 | 12 µs | 4.2 % |
STAMINA
STAMINA from Lambert Instruments is, like LIFA, a complete system. However, up to nine cameras can be used simultaneously. At the same time, the user-friendly software is compatible with a wide range of cameras and offers the option of easily integrating your own cameras into the imaging system.
STAMINA can also be inversely integrated into existing setups using API (Application Programming Interface) with compatible platforms MATLAB, LabView or Python3 (64-bit).
STAMINA synchronizes recordings so that all imaging data is streamed directly to disk, making only disk space a limitation.
Almost any camera can be integrated into the STAMINA setup, both an in-house Lambert Instruments product and an existing camera.