Fluorescence camera HiCAM Fluo

HiCAM Fluo from Lambert Instruments is a particularly powerful yet compact fluorescence camera that is connected to fluorescence microscopes.
Its exposure time can be adjusted down to the nanosecond range to image even fastest phenomena. By using high-quality Gen III amplifiers (18 or 25 mm), imaging down to the detection of single photons is possible. The amplifiers can be adapted to the respective application (UV to NIR) by a number of possible photocathodes.

HiCAM Fluo can also be used as a high-speed shutter by gating. This reduces the effective exposure time and allows motion blur to be reduced. The fanless cooling system, with its virtually non-existent vibrations, also ensures an improved signal-to-noise ratio and thus optimum image quality.

Thanks to its compact aluminum housing, the HiCAM Fluo is very handy and, with the help of its universal connections, it can be used with basically any fluorescence microscope. The images are streamed directly via a CXP (CoaXPress) interface with four CXS connections (6.25 Gbit/s each). The associated software not only controls the camera and measurement parameters, but also data processing, amplifier power and gating.


  • Combustion analysis
  • Cell medicine
  • Microbiology
  • Chip manufacturing
  • Microfluidics
  • LIF (laser-induced Fluorescence)
  • MTV (molecular tagging velocimetry)


Camera specifications
HiCAM Fluo 1000HiCAM Fluo 2000
Max. resolution1280 × 1024 pixels1920 × 1080 pixels
Frame rate1000 fps at max. resolution
1500 fps at 1200 × 720 pixels
4000 fps at 640 × 480 pixels
7500 fps at 256 × 256 pixels
2000 fps at max. resolution
2500 fps at 1280 × 960 pixels
5100 fps at 640 × 480 pixels
6400 fps at 512 × 384 pixels
Pixel size6.6 µm10 µm
Pixel depth10 or 12 bit8 bit
Computer interfaceStreaming CoaXPressStreaming CoaXPress 2.0
Integrated featuresGain and gain control
Anode current limiter
Internal trigger generator
Shutter control

Gating properties
standardoptionalprecision gating
Gating pulse width range40 - 10 ns<3 - 10 ns3 ns - 1 µs
Min. pulse width (jitter)40 ns (<250 ps RMS)<3 ns (<80 ps RMS)3 ns (<250 ps RMS)
Pulse width increments10 ns10 ps250 ps
pulse repetition rate100 kHz300 kHz, 2.5 MHz in burst mode

Intensifier properties
Image intensifierProximity-focused Gen III GaAsP (filmless)
Photon gain (max.)36,000 lm/m2/lx
(P20, P43 or FS on request,
see also their own specifications table)
Input WindowBorosilicate glass
Spatial resolution (bare intensifier)Up to 62.5 lp/mm
Spectral responses of phosphors
18 mm Gen III intensifier25 mm Gen III intensifier
Effective area on input12.78 × 12.68 mm17.75 × 17.61 mm
Input window thickness5.5 mm6.0 mm

Phosphor characteristics
PhosphorPeak wavelengthPhotons/electrons
@5 kV
Decay time
to 10 %
Screen efficiency
(Optical/Electrical Watt)
P46548 nm2401.3 ms8.7 %
P43530 nm55300 ns1.8 %
FS513 nm
668 nm
768 nm
9612 µs4.2 %


STAMINA from Lambert Instruments is, like LIFA, a complete system. However, up to nine cameras can be used simultaneously. At the same time, the user-friendly software is compatible with a wide range of cameras and offers the option of easily integrating your own cameras into the imaging system.
STAMINA can also be inversely integrated into existing setups using API (Application Programming Interface) with compatible platforms MATLAB, LabView or Python3 (64-bit).
STAMINA synchronizes recordings so that all imaging data is streamed directly to disk, making only disk space a limitation.
Almost any camera can be integrated into the STAMINA setup, both an in-house Lambert Instruments product and an existing camera.